USE OF A NATIVE EPITOPE FOR SELECTING EVOLVED BINDING MEMBERS FROM A LIBRARY OF MUTANTS OF A PROTEIN CAPABLE OF BINDING TO SAID EPITOPE

摘要

The invention provides a method for selecting at least one member from alibrary of proteinaceous molecules comprising providing at least one celland/or a functional equivalent thererof, with at least part of said libraryunder conditions that allow binding of any such member to an epitope in and/oron said cells and/or said functional equivalent thereof, removing unboundproteinaceous molecules and selecting said at least one member, wherein saidlibrary comprises at least one mutant of a proteinaceous molecule capable ofbinding to said epitope.