DEVICE AND METHOD FOR TREATING A SAMPLE BY OVERPRESSURED LAYER CHROMATOGRAPHY A STATIONARY PHASE, UNDER CONTROLLED FORCED FLOW

摘要

a device for processing sample by separation under forced flow(1) includes a chamber housing (2) with a stationary phase in athe first area (6) of a sample to be treated, means of pressure(8) an external surface (5) of the stationary phase, how todistribution (10) of a mobile phase at a second location (7) of the phase.a stationary inlet (13), a power output and a mobile phase.(18) for evacuation of the mobile phase, out of the house. the devicealso includes means (23) for the input stage (13)mobile, a sensor (29) for measuring the pressure of the upstream stage mobilethe inlet, a valve (31) positioned downstream of the outlet to control thethe flow rate of the mobile phase at the outlet of the chamber, and a module (25)control valve as a function of a comparison between the measured pressure anda pressure limiting valve (31) is selected and is opened when the pressurethe measured pressure is greater than or equal to the limit.