METHOD OF DETECTING DNA BY DNA HYBRIDIZATION METHOD WITH THEUSE OF FLUORESCENT RESONANCE ENERGY TRANSFER

摘要

A practically usable DNA detection method by a DNA hybridization method with the use of fluorescent resonance energy transfer wherein the background luminescence (i.e., luminescence based on the luminescence of a donor and th e direct excitation of an acceptor) affecting the sensitized luminescence of t he acceptor is minimized so that a target DNA strand can be conveniently and ve ry easily detected in a homogeneous solution. In this DNA detection method, a D NA is detected by the DNA hybridization method by using, as detection reagents, streptoavidin labeled with a fluorescent rare earth metal complex, a nucleic acid probe modified with biotin and a nucleic acid probe labeled with an organic cyanine pigment and taking advantage of fluorescent resonance energy transfer.