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METHOD FOR ISOLATING PROTEINS OR PROTEIN AND NUCLEIC ACID ASSOCIATIONS, AND RESULTING COMPLEXES OF PARTICLES AND PROTEINS, REAGENTS AND APPLICATIONS
METHOD FOR ISOLATING PROTEINS OR PROTEIN AND NUCLEIC ACID ASSOCIATIONS, AND RESULTING COMPLEXES OF PARTICLES AND PROTEINS, REAGENTS AND APPLICATIONS
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机译:分离蛋白质或蛋白质和核酸的组合的方法,以及产生颗粒和蛋白质,试剂和应用的复合物的方法
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摘要
the process for isolation of proteins and / or a combination of protein andnucleic acids in a sample, comprises the steps that:the sample is in contact with the magnetic colloidal particleswith a heart and a shell in which the core is magnetic.and is coated by at least one polymer containing functional groupsx is selected from hydroxyl groups, thiol, amine, aldehyde, ester,anhydride acid chloride, carbonate, carbamate, and isocyanatemethods and mixtures thereof, and at least one fraction is reacted withother functional groups of the envelope, and the envelope isconsisting of a polymer containing functional groups or ionizable zz ', identical or different, selected from among amino acid groups.carboxylic acid anhydride, ester, aldehyde, thiol, disulfide,. alpha. -halogu00e9nocarbonyle, sulfonic acid, maleimide, isocyanate and isothiocyanate.partially react with the functional groups x of the heart.as a mixture, the mixture is incubated under conditionsset, and separate the protein mixture and / or associationsprotein and nucleic acid in the complex particlesmeasured by application of a magnetic field. the invention also relates toa complex of colloidal particles and a reactive proteinwith such a compound or colloidal particles.
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