Improved protocol for activation of oocytes

摘要

A novel protocol for activation of mammalian oocytes or nuclear transfer fusions that use a CDC2 or CDK2 specific inhibitor selected from roscovitine, iso-olomoucine, olomoucine, flavopiridol, and DMAP is provided. This protocol provides for more efficient formation of blastocysts which can be used to produce cloned offspring and embryonic stem cells.