Method and device for the production of microparticles for controlled release of water-soluble pharmaceuticals and viralvectors. Application to the administration of plasmid DNA and defective recombinant adenovirus

摘要

The method involves obtaining a multiple emulsion by injecting immiscible liquid phases under high turbulence. The device used for said purpose, which is called TROMS or "Total Recirculation One-Machine System", comprises a pumping system (1) that is connected to a Rehodine valve (2) from which two needles (3, 4) having different inner diameters stick out. The first needle is inserted into the first mixing vessel (5) and the other needle is inserted into the second mixing vessel (6). The organic phase is introduced into the system by means of a glass syringe with a Teflon plunger (7). The mixing vessels are connected to the pump by two valves (8 and 9). The method and device used are suitable for the production of homogenous microcapsule or heterogeneous microsphere type microparticles that are suitable for the encapsulation of water-soluble pharmaceuticals including plasmid DNA, RNA, genes, oligonucleotides, peptides, proteins and viral vectors used in genic therapy and defective recombinant adenovirus.