Sample cuvette for measurements of total internal reflection fluorescence

摘要

A biochemical sample chamber (6) is made of a transparent material. At least two of the inner chamber walls (2, 3) act as substrates and are provided with catchers (5), such as chemical or affinity reagents. In use, the catchers are coupled to fluorescent groups (4) within the sample in a pattern representative for the sample. The fluorescent groups (4) of each chamber wall (2, 3) are excited, using total internal reflection technique, by light introduced to prisms (7, 8) corresponding to each inner wall which has been provided with catchers. The excited fluorescent groups emit light that is detected. Thus, the inner space of the chamber is used with high efficiency, thereby enabling an analizing capacity which is least twice that of a single chamber.