Method for generating resistance against cgmmv in plants, genetic constructs for use in said method, and cgmmv-resistant plants obtained via said method

摘要

The present invention relates to a method for generating resistance against Cucumber Green Mottle Mosaic Virus (CGMMV) in plants, in particular in plants susceptible to infection by CGMMV, such as iCucurbitaceae/i species, including melon, cucumber, watermelon and bottlegourd. In the invention, the plants are provided with replicase-mediated resistance against CGMMV. For this purpose, the plants are transformed with a polynucleotide sequence that: i) has been derived from the 129 kD sequence, the 57 kD sequence, or the 186 kD readthrough sequence of native CGMMV; ii) upon (at least) transformation into the plant and transcription into RNA - and usually also translation into the corresponding encoded protein - can provide the plant with resistance against CGMMV; but iii) does not encode any replicase activity. In particular, this will be a nucleotide sequence that encodes a defective variant of the replicase of CGMMV. The invention further relates to genetic constructs containing such a polynucleotide sequence suitable for use in the above method, and to CGMMV-resistant transgenic plants obtained via said method.