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D-gluconolactone oxidase gene and method for producing recombinant d-gluconolactone oxidase
D-gluconolactone oxidase gene and method for producing recombinant d-gluconolactone oxidase
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机译:D-葡萄糖酸内酯氧化酶基因及重组d-葡萄糖酸内酯氧化酶的生产方法
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摘要
The present invention is directed to the isolation of nucleic acid molecules which encode the enzyme D-gluconolactone oxidase (D-GLO) useful in the production of erythorbic acid by the conversion of D-gluconolactone. Various modifications of such nucleic acid molecules are contemplated including the encoded proteins which retain the enzymatic activity of naturally occuring D-GLO. Recombinant methods of producing D-gluconolactone oxidase utilizing the nucleic acids of this invention in various host cells transformed by appropriate expression vectors are preferred. Methods of utilizing the D-GLO of the present invention in processes for the conversion of glucose and specifically the conversion of D-gluconolactone to erythorbic acid are also contemplated.
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