IDENTIFICATION OF A VACCINE CANDIDATE FROM AN EXTRAINTESTINAL ISOLATE OF E. COLI

摘要

The present invention is directed to identifying a gene, iroNec, from an extraintestinal isolate of E. coli, CP9. This gene was identified from CP9 by screening a library of 527 mutant derivatives of CP9 with active TnphoA fusion in human urine. Two mutant derivative CP9.45 and CP9.82 possessed increased PhoA activity in urine due to the TnphoA insertion into iroNec. The product of this gene is an extracytoplasmic protein of 725 amino acids and can be used as vaccine against extraintestinal E. coli infections.