PROCESS FOR MANUFACTURING CYCLOSPORIN A BY HIGHLY PRODUCTIVE FUSANT STRAIN

摘要

The present invention relates to a process for making a highly productive fusant of Tolypocladium inflatum, a producing strain of cyclosporin A with immunosuppressive property wherein the selection of the fusant KD461, designed to produce a large amount of cyclosporin A, was made available by the following steps of: developping amino acid-dependent mutant of Tolypocladium inflatum, wild strain isolated from soil, which induced by UV radiation: conjugating L-valine-dependent and L-leucine-dependent mutant to promote the demand and utility of L-valine and L-leucine, precursors of cyclosporin A, together with organic nitrogen-source. The fusant KD461 has following characteristics in comparison with wild strain: a) in a solid medium, slowly growing in a malt-yeast extract agar medium, b) darker as light grey color in colony, c) backside of colony with dark brown, d) in a liquid medium short and thick hyphae with many arthrospore.