25-hydroxyvitamin D3-1a-hydroxylase and DNA encoding the hydroxylase

摘要

The present invention relates to a polypeptide having 25-hydroxyvitamin D₁-1α-hydroxylase activity, being useful for the prevention, diagnosis and therapeutic treatment of adult diseases such as osteoporosis induced by the decrease of active type vitamin D₁ and catalyzing the final stage of vitamin D₁ activation; and the gene encoding the polypeptide.;In accordance with the present invention, the following can be provided; a polypeptide having 25-hydroxyvitamin D₁-1α-hydroxylase activity, DNA encoding the polypeptide, a recombinant DNA prepared by inserting the DNA in a vector, a transformant carrying the recombinant DNA, a method for preparing 25-hydroxyvitamin D₁-1α-hydroxylase by using the transformant, a method for preparing 1α, 25-dihydroxyvitamin D₁ comprising using the polypeptide having 25-hydroxyvitamin D₁-1α-hydroxylase activity, and an antibody recognizing the polypeptide.