FUNCTIONAL VITAMIN D DERIVATIVES AND A METHOD FOR DETERMINING 25-HYDROXY-VITAMIN D AND 1ALPHA,25-DIHYDROXY-VITAMIN D

摘要

The invention relates to a multifunctional vitamin D derivative of formula (I) wherein; O represents the oxygen atom of an ether group; X represents a spacer group having a length of 0.8 to 4.2 nm, for example, an amino carboxylic acid radical, an amino undecanoic acid radical, or an amino polyether radical; Y represents hydrogen or hydroxy; A represents a tracer group such as biotin, digoxygenin or another vitamin D group which are bound by a protein having a higher affinity; R represents a hydrocarbon side-group of vitamin D or vitamin D metabolites. The invention also relates to a method for quantitatively determining a 25-hydroxy-vitamin D metabolite and a 1α,25-dihydroxy-vitamin D metabolite in a sample.