Expression system containing chimeric promoters with binding sites for recombinant transcription factors

摘要

The present invention relates to nucleic acid constructs characterized in that they comprise the following components:;Component a): one or more promoters,;Component b): b1) DNA encoding a DNA-binding domain and;b2) Glutamine, serine and threonine rich transactivation domains;Encrypting DNA; RTI ID = 0.0 a) /RTI ;DNA encoding the recombinant activator,;Component c): one or more DNA sequences for binding the expression product of component b);Component d): a CDE-CHR element in which the 5 'terminus is bonded to the 3 ' end of component c);One or more minimal promoters comprising E2FBS-CHR elements,;Component e): The transcription is activated by the expression product of component b) which binds to component c);Wherein the nucleic acid construct comprises at least one action factor gene.;The present invention also relates to the production and use of cells comprising nucleic acid constructs, nucleic acid construct containing vectors, vectors, and to the use of nucleic acid constructs in the manufacture of a medicament.