EXPRESSION SYSTEMS COMPRISING CHIMERIC PROMOTERS WITH BINDING SITES FOR RECOMBINANT TRANSCRIPTION FACTORS

摘要

Expression systems comprising chimeric promoters with binding sites for recombinant transcription factors The present invention relates to a nucleic acid construct which comprises the following components: ;Component a): at least one promoter ;Component b): a nucleic acid sequence encoding at least one recombinant transactivator whose transcription is activated by component a) and which comprises: ;component b1): a nucleic acid sequence encoding a DNA-binding domain ;component b2): a nucleic acid sequence encoding a transactivation domain comprising glutamine, serine and threonine ;Component c): at least one a nucleic acid sequence sequence for binding the expression product of component b) ;Component d): at least one promoter which comprises the CDE-CHR element or the E2FBS-CHR element and whose 5 end is bound, i.e., linked, to the 3 end of component c) ;Component e): at least one effector gene whose transcription is activated by the expression product of component b) binding to component c); ;to its preparation and its use; to vectors comprising the nucleic acid construct, cells comprising these vectors, and to the use of the nucleic acid construct for the preparation of a medicament.