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DIAGNOSIS REAGENT FOR DETECTING MYCOBACTERIUM TUBERCULOSIS COMPLEX AND MYCOBACTERIA OTHER THAN TUBERCLE BACILLI(MOTT)
DIAGNOSIS REAGENT FOR DETECTING MYCOBACTERIUM TUBERCULOSIS COMPLEX AND MYCOBACTERIA OTHER THAN TUBERCLE BACILLI(MOTT)
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机译:除结核杆菌(MOTT)以外,还用于检测结核分枝杆菌复合体和分支杆菌的诊断试剂
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摘要
PURPOSE: A diagnosis reagent for detecting Mycobacterium tuberculosis complex and Mycobacteria other than Tubercle bacilli(MOTT) and a diagnosing method of them using the diagnosis reagent are provided, thereby simply and specifically diagnosing Mycobacterium tuberculosis complex and Mycobacteria other than Tubercle bacilli(MOTT). CONSTITUTION: The diagnosis reagent containing a pair of duplex-PCR primers as an active ingredient amplifies 136 bp of DNA fragment for Mycobacterium Tuberculosis complex and 204 bp of DNA fragment for Mycobacteria other than Tubercle bacilli, wherein a pair of duplex-PCR primers binds rpoB gene encoding RNA polymerase beta subunit. The diagnosis reagent containing a pair of duplex-PCR primers as an active ingredient amplifies 235 bp of DNA fragment for Mycobacterium Tuberculosis complex and 136 bp of DNA fragment for Mycobacteria other than Tubercle bacilli, wherein a pair of duplex-PCR primers binds rpoB gene encoding RNA polymerase beta subunit.
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机译:目的:提供一种用于检测结核分枝杆菌复合体和结核分枝杆菌(MOTT)以外的诊断试剂,以及使用该诊断试剂的诊断方法,从而可以简单,明确地诊断结核分枝杆菌(MOTT)以外的结核分枝杆菌复合体和分枝杆菌。组成:诊断试剂含有一对双链PCR引物作为活性成分,可扩增结核分枝杆菌复合体的136 bp DNA片段和结核杆菌以外的分枝杆菌的204 bp DNA片段,其中一对双链PCR引物结合rpoB编码RNA聚合酶β亚基的基因。含有一对双链PCR引物作为活性成分的诊断试剂可扩增结核分枝杆菌复合体的235 bp DNA片段和结核杆菌以外的分枝杆菌的136 bp DNA片段,其中一对双链PCR引物结合编码rpoB基因的RNA聚合酶β亚基。
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