METHOD OF SYNTHESIS OF POLYPEPTIDE ELICITING ANTIGENIC ACTIVITY OF HEPATITIS E VIRUS AND SET FOR ASSAY OF ANTIBODY RAISED TO HEPATITIS E PATHOGEN

摘要

FIELD: medicine, molecular biology, genetic engineering of proteins. SUBSTANCE: invention proposes a method for preparing recombinant polypeptides for their using in set for assay of antibodies raised to hepatitis E virus. Set has series of recombinant antigens synthesized in E. coli cells transformed with recombinant DNAs. Recombinant DNAs involve fragment of the second and the third open reading frames of hepatitis E virus genome. Sets can contain E. coli β-galactosidase as a control antigen. EFFECT: enhanced sensitivity and specificity in assay of spectrum of antibodies to individual viral proteins. 6 cl, 2 tbl, 13 ex