METHOD OF CULTIVATION, FIBROBLASTS, METHOD OF CELL SEPARATION

摘要

FIELD: biotechnology. SUBSTANCE: cultivation of human stem cells is carried out ex vivo in liquid cultural medium with medium exchange rate 50-100% of every day change at cell density 1 x 10⁴ - 1 x 10⁷/ ml of medium. Cultivation is carried out in the presence of growth factors and/or transformed stromal cells secreting growth factor. Transfer vector of gene providing reading the stable genetic information is added to culturing medium additionally. For separation of neoplastic cells from normal ones hemopoietic cells combined with stromal cells are subjected for liquid flow making shear stress at value 1.0 dyne/cm² or above. Invention can be used for hemopoietic cell cultures growing and to optimize growing stem cells and cell-precursors. EFFECT: improved method of cultivation and separation of cells. 21 cl, 9 dwg, 7 tbl