METHOD OF PREPARING VACCINE BCG 'IMURON' FOR BLADDER CANCER IMMUNOTHERAPY

摘要

FIELD: medicine, oncology, medicinal microbiology. SUBSTANCE: bacterial mass of culture BCG-1 is filtered and diluted with stabilizing agent for two stages. After the first dilution up to concentration 50-60 mg of BCG in 1 ml the obtained suspension is centrifuged at separation factor 250 for 5 min and then the second dilution is carried out up to concentration 25 or 30 mg of BCG in 1 ml. Vaccine is poured into ampoules in the dose 1 ml and lyophilized. Invention provides to obtain standard curative vaccine with concentration 25 or 30 mg of BCG in 1 ml. EFFECT: enhanced indices of viability, homogeneity, dispersity and solubility. 2 tbl, 3 ex _