Process for the pure presentation of the protease activating the blood coagulation factor VII, its proenzyme or a mixture of both proteins by means of ion exchange chromatography

摘要

A method is described for the purification of the protease activating the blood coagulation factor VII and / or its proenzyme, in which it is produced from biological or DOLLAR A) liquids produced by genetic engineering by means of anion or cation exchange chromatography at a pH below the isoelectric Point of the protein to be isolated or DOLLAR A b) by a combination of anion or cation exchange chromatography with affinity chromatography and / or fractional precipitation at pH values between 2.5 and 9.0, preferably between 2.5 and 7, 2 can be obtained, the affinity chromatography using DOLLAR A - calcium phosphate / hydroxylapatite, DOLLAR A - a hydrophobic matrix, DOLLAR A - a chelating matrix, DOLLAR A - a matrix which is combined with an immobilized or directed against the protein to be isolated, monoclonal or polyclonal antibody or its F (ab) or F (ab) 2 fragments ten coated is carried out. DOLLAR A A pharmaceutical preparation and a reagent are also described which contain the protease and / or its proenzyme.