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Production of F(ab)2 fragments of monoclonal antibodies without isolating the antibodies, comprises dialysis of hybridoma culture supernatant, first with an acid buffer and then with a neutral salt buffer
Production of F(ab)2 fragments of monoclonal antibodies without isolating the antibodies, comprises dialysis of hybridoma culture supernatant, first with an acid buffer and then with a neutral salt buffer
Producing immunoreactive F(ab)2 fragments of monoclonal antibodies, comprising subjecting culture supernatant from in vitro or ascites hybridoma culture containing endogenous or added procathepsin D to dialysis at 4-10 deg C, against a pH 2.5-4.0 buffer to activate the procathepsin D, and then against a salt-containing pH 7.0-8.2 buffer to stop the proteolysis after antibody processing, is new.
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