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Oligomer - an array with a pna - and / or dna - oligomers on a surface

机译:齐聚物-表面具有pna和/或dna齐聚物的阵列

摘要

The invention relates to an oligomer array with PNA (Peptide Nucleic Acid) and/or DNA oligomers on a surface, said oligomers comprising oligomers of 6 to 20 monomers or nucleobases each. Said monomers or nucleobases comprise at least one sequence of the general formula DDCGDD or of the general formula DDTGDD or of the general formula HHCGHH or of the general formula HHCAHH, wherein H represents any of the bases adenine (A), cytosine (C) or thymine (T) and D represents any of the bases adenine (A), guanine (G) or thymine (T). The location of the oligomers on the surface is correlated with the sequence of the oligomers. The inventive oligomer arrays are used to detect cytosine methylations in genomic DNA.
机译:本发明涉及在表面上具有PNA(肽核酸)和/或DNA低聚物的低聚物阵列,所述低聚物包括6至20个单体或核碱基的低聚物。所述单体或核碱基包含通式DDCGDD或通式DDTGDD或通式HHCGHH或通式HHCAHH的至少一个序列,其中H代表腺嘌呤(A),胞嘧啶(C)或胸腺嘧啶(T)和D代表任何腺嘌呤(A),鸟嘌呤(G)或胸腺嘧啶(T)。低聚物在表面上的位置与低聚物的序列相关。本发明的低聚物阵列用于检测基因组DNA中的胞嘧啶甲基化。

著录项

  • 公开/公告号DE19957827A1

    专利类型

  • 公开/公告日2001-06-21

    原文格式PDF

  • 申请/专利权人 EPIGENOMICS AG;

    申请/专利号DE1999157827

  • 发明设计人 BERLIN KURT;

    申请日1999-11-25

  • 分类号C07H21/00;C07H1/00;C12P19/34;G01N33/48;C12Q1/68;C07K17/00;H01J49/02;

  • 国家 DE

  • 入库时间 2022-08-22 01:10:07

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