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New nucleic acid encoding nitrilase, useful for converting aromatic nitriles to carboxylic acid, pharmaceutical intermediates, isolated from Rhodococcus rhodochrous
New nucleic acid encoding nitrilase, useful for converting aromatic nitriles to carboxylic acid, pharmaceutical intermediates, isolated from Rhodococcus rhodochrous
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机译:编码腈水解酶的新核酸,可用于将芳香腈转化为羧酸,药用中间体,从红球红球菌中分离出来
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摘要
Isolated nucleic acid (I), encoding a polypeptide (II) with nitrilase activity, is (i) a 1483 bp sequence (1), reproduced; (ii) a sequence equivalent to (1) within the degeneracy of the genetic code or (iii) a derivative of (1) that encodes a polypeptide having at least 95% homology with a 366 amino acid polypeptide (2), reproduced, and essentially the same enzymatic activity as (2). Independent claims are also included for the following: (a) (II) encoded by (I); (b) nucleic acid construct containing (I) linked to one or more regulatory signals; (c) vector containing (I) or the construct of (b); (d) recombinant microorganism containing (I), the construct of (b) or the vector of (c); and (e) method for producing achiral or chiral carboxylic acids (III) by treating nitriles (IV) with (II) or with growing, resting or lysed microorganisms of (d).
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