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Ligase / polymerase - a method for the detection of cytosine - methylation in dna samples
Ligase / polymerase - a method for the detection of cytosine - methylation in dna samples
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机译:连接酶/聚合酶-一种检测dna样品中胞嘧啶甲基化的方法
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摘要
The invention relates to a method for detecting 5-methylcytosine in genomic DNA samples. Firstly, a genomic DNA from a DNA sample is chemically reacted with a reagent, whereby 5-methylcytosine and cytosine react differently. Afterwards, the pretreated DNA is amplified while using a polymerase and at least one primer. In the next step, the amplified genomic DNA is hybridized to at least two oligonucleotides, whereby the latter are assembled by inserting at least one oligonucleotide. In the case of the ligation product, a nucleotide carries a detectable tagging, and the lengthening is subject to the methylation status of the respective cytosine in the genomic DNA sample. In the following step, the lengthened oligonucleotides are examined for the presence of the tagging.
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