Novel electron donor system useful for the production of omega-hydroxylated fatty acids comprises an inorganic electron sink and a mediator which enables the electron transfer

摘要

An electron donor system (M1) that transfers electrons to an enzyme with redox properties comprising an inorganic, not electrode-bound, electron sink and a mediator which enable the electron transfer is new. Independent claims are also included for: (1) transferring (M2) oxygen to a hydrocarbon containing hydrogen donor molecule, where the hydrogen donor molecule is in a reaction medium comprising the oxygen transferring enzyme and (M1) in the presence of oxygen and incubating under suitable reaction conditions; (2) the enzymatic production (M3) of terminally or subterminally hydroxylated (position w-1 to w-4) fatty acids comprising: (i) mixing a hydroxylatable fatty acid or fatty acid derivative in the presence of (M1) and cytochrome P450 monooxygenase; and (ii) isolating the hydroxylated product; (3) a bioreactor useful for the production of w-hydroxylated fatty acids as described in (2); and (4) detecting (M4) fatty acid-monooxygenases comprising: (i) contacting the analyte with a w-hydroxylatable fatty acid or a derivative comprising a terminal chromophore or fluorescent label in (M1); and (ii) qualitatively or quantitatively detecting the signal.