Determining genetic predisposition to disease, e.g. rheumatoid polyarthritis, by reacting amplicons of polymorphic human leukocyte antigen (HLA) region with both high and low resolution probes

摘要

Analyzing the genetic predisposition of a subject to diseases comprising treating a liquid sample containing - 1 type of amplicon from - 1 polymorphic region associated with the disease, with - 1 type-specific probe (P1, low resolution) and - 1 subtype-specific probe (P2, high resolution), is new. Analyzing the genetic predisposition of a subject to diseases comprising treating a liquid sample containing - 1 type of amplicon from - 1 polymorphic region associated with the disease, with - 1 type-specific probe (P1, low resolution) and - 1 subtype-specific probe (P2, high resolution), is new. P1 hybridizes to the polymorphic region of - 1 gene, or group of alleles, present on the amplicon while P2 hybridizes to the allele, or group of alleles, specifically recognized by P1. P2 can discriminate between alleles associated with susceptibility and/or resistance to the disease, according to whether they hybridize or not.