Analyzing genetic predisposition to disease, e.g. rheumatoid polyarthritis, by amplification then hybridization to low- and high-resolution oligonucleotide probes

摘要

Analyzing genetic predisposition to at least one disease, comprising treating a liquid sample, containing at least one type of amplicon derived from at least one polymorphic region (PMR) related to the disease, with at least one type-specific probe (P1; low resolution), and at least one subtype-specific probe (P2; high resolution), is new. Analyzing genetic predisposition to at least one disease, comprising treating a liquid sample, containing at least one type of amplicon derived from at least one polymorphic region (PMR) related to the disease, with at least one type-specific probe (P1; low resolution), and at least one subtype-specific probe (P2; high resolution), is new. P1 hybridizes to at least one gene, or a group of alleles of the gene, present in the amplicon and P2 hybridizes to the allele, or group of alleles, specific to P1. P2 can discriminate between one or more alleles associated with susceptibility and/or those alleles associated with resistance to disease, according to whether they hybridize or not. Independent claims are also included for the following: (1) amplification of a sequence corresponding to the group of DRB1 asterisk gr04 alleles using primers (20) and (21); (2) amplification of a sequence corresponding to the B27 allele using primers (22), (23) and/or (25) plus (24) and/or (26); and (3) combined amplification products of (1) and (2). CCGGATCCTTCGTGTCCCCACAGCACG (20); TCGCCGCTGCACTGTGAAG (21); GGGAGGAGCGAGGGGACCCCAG (22); GGGAGGAGCGAGGGGACCGCAG (23); ATCTCGGACCCGGAGACT (24); AGGGGACCGCA (25); and ATCTCGGACCCGGAGACTCG (26).