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Pseudomonas chlororaphis microorganism, polyurethane degrading enzyme obtained therefrom, and method of use
Pseudomonas chlororaphis microorganism, polyurethane degrading enzyme obtained therefrom, and method of use
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机译:绿假单胞菌微生物,由其获得的聚氨酯降解酶及其使用方法
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摘要
Wild-type bacteria with polyurethanase activity, and the ability to excrete polyurethanase into a culture medium have been genetically altered to significantly increase their polyurethanase activity. Biologically pure cultures of these genetically altered are cultured in a medium containing an inducer for the production of polyurethanase. The bacteria are then removed from the culture medium to provide a preparation with a high polyurethanase activity. One useful genetically altered bacteria is a strain of Pseudomonas chlororaphis designated ATTC No. 55729. The polyurethanase-containing preparation may then be used directly to degrade polyurethane, particularly polyurethane and polyurethane-based coatings.
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