Method and apparatus for analyzing data files derived from emission spectra from fluorophore tagged nucleotides

摘要

An automated method and apparatus is provided for the analyzing of data files derived from fluorophore emissions detected during observation of fluorophore labeled nucleotide polymers such as is done during the sequencing of bases in nucleotide polymers. The analysis steps of the method depend upon a key step of quantifying features of the emission peaks whereby subsequent steps as base calling can be performed and whereby individual emission spectra within the data files of two or more samples can be automatically synchronized, compared, and differences detected and signaled. The quantified peak information provides for the use of fuzzy logic and the assignment of truth values or scores to be assigned to the base calls. optimally, individual peaks within the data file are corrected by distortions, the peaks enhanced, and the overall data file information augmented to further improve the accuracy of the analysis of the data and reduce manual labor requirements.