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Solid phase synthesis of oligonucleotides using carbonate protecting groups and alpha-effect nucleophile deprotection
Solid phase synthesis of oligonucleotides using carbonate protecting groups and alpha-effect nucleophile deprotection
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机译:使用碳酸盐保护基和α效应亲核试剂脱保护进行寡核苷酸的固相合成
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摘要
The invention provides a method for synthesizing oligonucleotides using carbonate protection of hydroxyl groups and nucleophilic deprotection reagents. The deprotection reagents irreversibly cleave the carbonate protecting groups while simultaneously oxidizing the intemucleotide phosphite triester linkage, and can be used in aqueous solution at neutral to mildly basic pH. The method eliminates the need for separate deprotection and oxidation steps, and, since the use of acid to remove protecting groups is unnecessary, acid-induced depurination is avoided. Fluorescent or other readily detectable carbonate protecting groups can be used, enabling monitoring of individual reaction steps during oligonucleotide synthesis. The invention is particularly useful in the highly parallel, microscale synthesis of oligonucleotides. Reagents and kits for carrying out the aforementioned method are provided as well.
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