Type II restriction endonuclease, HPY 188 III, obtainable from Helicobacter pylori J188 and a process for producing the same

摘要

In accordance with the present invention, there is provided a novel restriction endonuclease and its DNA obtainable from Helicobacter pylori J188 (NEB#1174), hereinafter referred to as “Hpy188III”, which endonuclease:;(1) recognizes the nucleotide sequence 5′-TCNNGA-3′ in a double-stranded DNA molecule as shown below,; $PTEXTPDAT5\′-TC\↓NNGA-3\′/PDAT/PTEXT$ ; $PTEXTPDAT3\′-AGNN\↑CT-5\′/PDAT/PTEXT$ ;(wherein G represents guanine, C represents cytosine, A represents adenine, T represents thymine and N represents either G, C, A, or T);;(2) cleaves said sequence in the phosphodiester bonds between the C and first unspecified nucleotide N as indicated with the arrows; and;(3) cleaves double-stranded PhiX174 DNA to produce 26 fragments, including fragments of 704, 560, 497, 477, 423, 309, 308, 304, 261, 225 basepairs, and 16 fragments smaller than 200 basepairs.