Type II restriction endonuclease, Hpy99I, obtainable from Helicobacter pylori J99 and a process for producing the same

摘要

In accordance with the present invention, there is provided a novel restriction endonuclease and its DNA obtainable from Helicobacter pylori J99 (NEB#1237), hereinafter referred to as “Hpy99I”, which endonuclease:;(1) recognizes the nucleotide sequence 5′-CGWCG-3′ in a double-stranded DNA;(wherein G represents guanine, C represents cytosine, A represents adenine, T represents thymine and W represents either A, or T);;(2) cleaves double-stranded PhiX174 DNA to produce 8 fragments, including fragments of 3063, 629, 602, 447, 389, and 176 base pairs, and 2 fragments smaller than 100 base pairs.