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Compositions and methods for determining the activity of DNA-binding proteins and of initiation of transcription
Compositions and methods for determining the activity of DNA-binding proteins and of initiation of transcription
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机译:用于确定DNA结合蛋白活性和转录起始的组合物和方法
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摘要
This invention provides plasmids that are useful in detecting and determining the DNA-binding activity of sequence-specific DNA-binding molecules. The invention further provides plasmids that are useful in detecting and determining the activity of RNA polymerases in initiating transcription. In particular, the invention relates to plasmids that contain unique restriction sites and cognate nucleotide recognition sequences for sequence-specific DNA-binding molecules. Also provided are methods for using the plasmids disclosed herein.
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