DNA molecule encoding interferon-gamma (IFN-) inducing factor (IGIF, IL-18) and DNA fragment thereof

摘要

A protein which induces the IFN- production by immunocompetent cells and has a molecular weight of 19,0005,000 daltons on SDS-PAGE or gel filtration method and a pI of 4.81.0 on chromatofocusing. The protein is isolated from mouse liver and can be purified by a monoclonal antibody specific to it. The monoclonal antibody can be also used for assaying the protein. Fragments of the protein can be used for production of antibodies, and can be produced recombinantly using appropriate medric acids, host cells and vectors. Fragments of nuclear acids may also be used as probes.