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Identification and characterization of interacting molecules due to the interaction junction automated
Identification and characterization of interacting molecules due to the interaction junction automated
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机译:相互作用连接的自动化识别和表征
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摘要
(57) [Abstract] The present invention, for identifying was designed to detect positive clones from false positive clones majority rather separated by two-hybrid system of the conventional interaction molecules, characterized optionally relates to an improved method of. The method of the present invention is based on the novel combination of selection steps used to detect clones expressing interacting molecules from false positive clones. Provides the (efficient) interaction screening high throughput to identify reliably interacting molecules, therefore, the present invention may be bound to the identification of agents that inhibit the interaction. Inhibitors of this type may be found in the preparation of pharmaceutical compositions and uses thereof. The present invention further relates to kits useful in practicing the method of the present invention.
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