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Identification and characterization of interacting molecules by automated interaction mating
Identification and characterization of interacting molecules by automated interaction mating
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机译:通过自动相互作用配对识别和表征相互作用分子
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摘要
The present invention relates to an improved method for the identification and optionally the characterization of interacting molecules designed to detect positive clones from the rather large numbers of false positive clones isolated by two-hybrid systems. The method of the invention relies on a novel combination of selection steps used to detect clones that express interacting molecules from false positive clones. The present invention further relates to a kit useful for carrying out the method of the invention. The present invention provides for parallel, high-throughput or automated interaction screens for the reliable identification of interacting molecules.
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