Co-culture of cells in the micropatterned form

摘要

(57) and a micro-pattern form on a substrate of cells at least two [Abstract], as a result, cells heterozygous tea pick - may adjust the synthesis and / or function cell metabolism by adjusting the degree of cell contact I to provide a method capable of producing a co-culture of cells. By coating the substrate with a protein such as collagen, the protein will define the micro-pattern of the substrate, the co-culture is contacted with the cells of the first type, such as hepatocytes of the coated substrate in the medium to make a micropatterned cells coated substrate can be bound to Tanpata cytoplasm the cells is, the substrate and the cells in contact with the cells of the second type, such as fibroblasts micropatterned substrate in a second medium It is possible that by binding, are performed. The second medium is a non-adhesive medium such as serum-free medium or the first medium and the other is an adhesive medium such as serum-containing medium. Adhesive medium may also contain adhesion factors such as fibronectin or antibody. The micro pattern can be formed in an island by one cell (island) either the second kind or the first type, the cell is surrounded by cells of the other species.