Method for measuring protein interactions - protein in living cells

摘要

(57) [Abstract] providing a cell comprising a second conjugate and heterogeneous first conjugate of (a) heterogeneous, where the first conjugate disparate first and interest conjugated to a detectable group The second joint of heterologous and consisting of protein consists of a second protein of interest conjugated to a protein which binds specifically to the internal structure of the cell; binding of the detectable groups to (b) Internal structure then consists in indicating that detects the presence, the presence of bound second protein and a first of interest is specifically bound to each other, a protein in living cells - method for detecting protein interactions. Nucleic acid encoding the fusion protein, cells which contain and express such fusion proteins, another aspect of the invention, useful nucleic acid library as a screening tool for the implementation of the methods and kits useful in the practice of the method I include the. This invention is useful for screening compounds for the ability to destroy or inhibit the binding of a binding pair known, to identify its competitive inhibitor by it. This invention is useful to identify compounds that bind to the protein of interest known to screen for proteins known to one of interest for the library of other proteins of interest.