Homogeneous diagnostic assay that utilizes the simultaneous amplification of the signal and target

摘要

(57) [Abstract] The present invention relates to a reagent composition methods, and kits for determining the presence using a two-step cycling reaction target, the target nucleic acid in the sample. During one stage of the reaction cycle, and then hybridized with the probe nucleic acid, the target nucleic acid, causing the release of activator to the assay medium. During stage 2 of the reaction, the target analog - anchor complex is cleaved by the activator. This is, to achieve the dual purpose of that initiation of signal generation and release of target analog to the assay medium. Then, (which causes the release and the activator of the second) target analog liberated resumes reaction cycle by binding to the second probe. The reaction cycle results in an increase in the amount of signal generated from the target nucleic acid molecule of one sample. This enhances greatly the target detection level can be expected.