首页>
外国专利>
Cyanine-labeled dye and method for synthesizing the same, and cyanine-labeled protein and method for producing the same
Cyanine-labeled dye and method for synthesizing the same, and cyanine-labeled protein and method for producing the same
展开▼
机译:花青标记的染料及其合成方法,花青标记的蛋白质及其制备方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
PURPOSE: To obtain a new dye capable of binding with a protein such as an antibody and used for the preparation of a labeled antibody useful for the detection of an antigen by an immunochromatographic technique by succinimidylating the new carboxylic acid derivative of a cyanine-based compound. CONSTITUTION: This new cyanine-based carboxylic acid derivative expressed by formula II is obtained by reacting hydrazinobenzenesulfonic acid with isopropyl methyl ketone, then reacting a halogenized alkyl acid to give a carboxyalkylindoleniumsulfonate expressed by formula I (M is H or an alkali metal; n is 1 4; X is a halogen), then dissolving into pyridine after forming its potassium salt, reacting with an orthoformic acid ester while refluxing under an argon gas flow. Next, by reacting this compound with hydroxysuccinic imide in dimethylformamide in the presence of a condensation agent, a cyanine-based labeling pigment expressed by formula III, capable of binding with a protein such as an antibody to give a highly water soluble cyanine labeled protein, is obtained.
展开▼