Native chemical ligation of the solid phase of the protected peptide in N- terminal cysteine ​​or unprotected in Aqueous Solution

摘要

(57) [Abstract] The present invention, there is provided a kit method and apparatus for synthesizing the proteins and peptides assembled on the solid phase, the synthesis, using a mild linking chemical in aqueous solution , it is to be continuously connected to the peptide segments unprotected three or more. It is another object of the present invention also provides a method of using electron ionization mass spectrometry or MALDI, to monitor the solid phase sequential ligation.