PRESERVATION OF LILY IN TEST TUBE BY DARK CULTURE, GROWTH AND METHOD FOR CULTIVATING BULB

摘要

PROBLEM TO BE SOLVED: To develop a method for simply preserving bulbs of lilies (Lilium, Allium and Tulipa) in a sterile condition for a long time by tissue culture without using a large culture space and labor while planning to enlarge and grow the bulbs. SOLUTION: The method is to simply preserve bulbs of lilies (Lilium, Allium and Tulipa) in a dark and sterile condition for a long time by tissue culture at a high temperature which is higher than the germination temperature and 1-2 deg.C lower than the lethal temperature while enhancing the concentration of a medium to be remarkably higher than that of the usual one. The method is to enlarge the bulbs by increasing the concentrations of inorganic salts to 2-7 times and those of sugars to 2-5 times. The method is to preserve the bulbs at a high temperature to grow small bulbs during preservation. The bulbs can be obtained collectively at a desired timing without repeating cultures until the yield when cultured bulbs are successively preserved by using this method.