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MONOCLONAL ANTIBODY RECOGNIZING NORWALK VIRUS(NV)

机译:单克隆抗体识别诺瓦克病毒(NV)

摘要

PROBLEM TO BE SOLVED: To solve the problem that although conventionally an antigen analysis of epidemic gastroenteritis ileal obstruction caused by Norwalk virus(NV) as a pathogen is mainly carried out by RT-RCR or a method by using an antibody recognizing a capsid protein in a beculovirus system, the former takes time for a complex operation, and in the latter, the detection of the virus over the group is difficult because of the high specificity of the antibody, and to provide a method for ready and rapid detection regardless of the genogroup. SOLUTION: An antibody capable of recognizing viruses belonging to both genogroups I and II is formed by using a protein obtained by the expression in an Escherichia coli system as an antigen, and the antibody is used for solving the problem.
机译:解决的问题:为了解决这样的问题,尽管通常以RT-RCR或通过使用识别衣壳蛋白的抗体的方法主要通过RT-RCR进行由诺沃克病毒(NV)引起的流行性肠胃炎回肠阻塞的抗原分析。在Beculovirus系统中,前者需要花费大量时间进行复杂的操作,而在后者中,由于抗体的高特异性,很难在整个组中检测病毒,并且无论哪种方法,都可以提供一种快速,简便的检测方法基因组。解决方案:能够识别属于基因组I和基因组II的病毒的抗体是通过使用在大肠杆菌系统中表达获得的蛋白质作为抗原形成的,该抗体用于解决该问题。

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