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Screening method for the discovery and directed evolution of oxygenase enzymes
Screening method for the discovery and directed evolution of oxygenase enzymes
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机译:氧化酶的发现和定向进化的筛选方法
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摘要
The present invention relates to screening methods for oxidation enzymes, particularly mono- and dioxygenases. According to the methods of the invention, a product of an oxidation reaction is converted into a phenol or a catechol, which is easily detected by a Gibbs assay. This conversion allows for a sensitive and efficient assay. Both high-throughput liquid-phase and solid-phase methods using these principles are provided. Also described are method for detecting phenolic ether-products and sulfhydryl products from oxidation reactions, also using a Gibbs assay.
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