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Cell culture system for determining the cholesterol efflux potential for serum

机译：用于确定血清胆固醇外流潜能的细胞培养系统

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摘要

The present invention relates to a cell culture system to provide a tool for assessing the potential of a patient's serum for preventing the accumulation of cholesterol in arteries (i.e. serum efflux potential) that leads to atherosclerosis and to screen new drug compositions being developed to reduce the accumulation of cholesterol or enhance the clearance of cholesterol from the vessel wall. The present invention combines two individual assays, which are two different cholesterol assays: an assay measuring scavenger receptor class B type I (SR-BI)-mediated cholesterol efflux and an assay measuring ATP binding cassette protein 1 (ABCA1)-mediated cholesterol efflux, and uses them in parallel to test human and animal sera for their potential to stimulate efflux, as mediated by either of the two receptors described above.

机译：本发明涉及一种细胞培养系统，其提供一种工具，该工具用于评估患者的血清预防胆固醇在动脉中积累的潜力（即血清外排潜力），该潜力可导致动脉粥样硬化，并筛选正在开发的药物组合物以减少动脉粥样硬化。胆固醇的积累或提高血管壁中胆固醇的清除率。本发明结合了两种单独的测定法，它们是两种不同的胆固醇测定法：一种测定I型清道夫受体介导的胆固醇流出的测定法和一种测定ATP结合盒蛋白1（ABCA 1 ）介导的胆固醇外排，并并行使用它们来测试人和动物血清刺激外排的潜力，这是由上述两种受体之一介导的。 展开▼

著录项

公开/公告号US2002146681A1

专利类型

公开/公告日2002-10-10

原文格式PDF

申请/专利权人 ROTHBLAT GEORGE H.;
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申请/专利号US20020096705

发明设计人 GEORGE H. ROTHBLAT;
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申请日2002-03-13

分类号C12Q1/60;C12Q1/00;

国家 US

入库时间 2022-08-22 00:49:49

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2. 用于人多潜能干(hPS)细胞的生长和分化的3D培养系统 [P] . 中国专利： CN102459574A . 2012-05-16

3. Cell culture system for determining the cholesterol efflux potential for serum [P] . 美国专利： US7029863B2 . 2006-04-18

机译：用于确定血清胆固醇外流潜能的细胞培养系统

4. Autologous serum-added bone marrow cell culture system, autoserum-added bone marrow cell culture method, and method for producing a pharmaceutical composition comprising autologous serum-added bone marrow cells as an active ingredient [P] . 日本专利： JPWO2012018040A1 . 2013-10-03

机译：自体添加血清骨髓细胞培养系统，自体添加血清骨髓细胞培养方法，以及以自体添加血清骨髓细胞为有效成分的药物组合物的制造方法

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机译：自体添加血清骨髓细胞培养系统，自体添加血清骨髓细胞培养方法，以及以自体添加血清骨髓细胞为有效成分的药物组合物的制造方法

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focus on the nitric oxide pathwayAging395Heart failure with preserved ejection fraction develops when feeding western diet to senescence-accelerated mice396Cardiovascular markers as predictors of cognitive decline in elderly hypertensive patients397Changes in connexin43 in old rats with volume overload chronic heart failureGenetics and epigenetics400Calcium content in the aortic valve is associated with 1G>2G matrix metalloproteinase 1 polymorphism401Neuropeptide receptor gene s (NPSR1) polymorphism and sleep disturbances402Endothelin-1 gene Lys198Asn polymorphism in men with essential hypertension complicated and uncomplicated with chronic heart failure403Association of common polymorphisms of the lipoprotein lipase and pon1 genes with the metabolic syndrome in a sample of community participantsGenomics, proteomics, metabolomics, lipidomics and glycomics405Gene expression quantification using multiplexed color-coded probe pairs to determine RNA content in sporadic cardiac myxoma406Large-scale phosphorylation study of the type 2 diabetic heart subjected to ischemia / reperfusion injury407Transcriptome-based identification of new anti-inflammatory properties of the olive oil hydroxytyrosol in vascular endothelial cell under basal and proinflammatory conditions408Gene polymorphisms combinations and risk of myocardial infarctionComputer modelling, bioinformatics and big data411Comparison of the repolarization reserve in three state-of-the-art models of the human ventricular action potentialMetabolism, diabetes mellitus and obesity414Endothelial monocyte-activating polypeptide-II improves heart function in type -I Diabetes mellitus415Admission glucose level is independent predictor of impaired left ventricular function in patients with acute myocardial infarction: a two dimensional speckle-tracking echocardiography study416Association between biochemical markers of lipid profile and inflammatory reaction and stiffness of the vascular wall in hypertensive patients with abdominal obesity417Multiple common co-morbidities produce left ventricular diastolic dysfunction associated with coronary microvascular dysfunction, oxidative stress and myocardial stiffening418Investigating the cardiovascular effects of antiretroviral drugs in a lean and high fat/sucrose diet rat model of obesity419Statins in the treatment of non-alcoholic steatohepatitis (NASH). 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