Assay of nucleic acid by fluorescence polarization technique and detection of verotoxin-producing microorganisms

摘要

A method for assaying a nucleic acid comprising amplifying a nucleic acid in a sample by gene amplification and measuring the increased amount of the nucleic acid by a fluorescence polarization technique, in which the gene amplification is carried out by asymmetric amplification, or annealing with the primers used in the amplification is carried out after amplification of the nucleic acid, thereby to facilitate binding of a fluorescence-labeled reagent to the target single-stranded nucleic acid.