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Methods and reagents for quantitation of HLA-DR and CD11b expression on peripheral blood cells
Methods and reagents for quantitation of HLA-DR and CD11b expression on peripheral blood cells
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机译:定量测定外周血细胞中HLA-DR和CD11b表达的方法和试剂
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摘要
Improved methods, reagents, and kits for quantitation of HLA-DR and/or CD11b expression on peripheral blood cells are presented. Inclusion of a lysosomotropic amine, such as chloroquine, during staining stabilizes HLA-DR and CD11b expression. Use of a novel anti-CD14 conjugate, anti-CD14-PerCP/CY5.5, permits the ready discrimination of monocytes. The improved methods, reagents, and kits may be used to assess immune competence, and to direct and monitor immunostimulatory therapies in septic patients exhibiting monocyte deactivation.
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