Fluorescence polarizationamp;mdash;based diagnostic assay for equine infectious anemia virus
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机译:马传染性贫血病毒的基于荧光偏振的诊断分析
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摘要
A fluorescence polarization assay for Equine Infectious Anemia Virus utilizes a short peptide reagent probe derived from a conserved immunodominant region of gp45. The probe is N-terminally labeled, preferably with 6-carboxyfluorescein, and purified by HPLC, which reacts in a homogenous assay with anti-EIAV antibodies contained in the serum of field infected horses and ponies. The assay has a sensitivity of about 90 percent with a specificity approaching 100 percent.
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