Fusion proteu00ecna P26, soluble in water, vu00ecrus of equine infectious anaemia, process for its preparation and kit for diagnosis of equine infectious anemia

摘要

Invention patent: "proteu00ecna fusion P26, soluble in water, vu00ecrus of equine infectious anaemia, process for its preparation and kit for diagnosis of equine infectious anaemia.The present invention relates to a fusion protein P26, soluble in water.The virus of infectious anaemia equ00b3ina (s) that covers the portion of the gag of P26 this virus and the sequ00b3u00eancia amino acids 1 to 200 of the enzyme glutathione s - transferase (GST) of Schistosoma japonicumAnd its use in kits for diagnosis of infectious anaemia equ00b3ina.The invention also refers,A process for the preparation of such protein comprising steps of isolation of the sequ00b3u00eancia gag DNA viruses of infectious anaemia equ00b3ina responsible for expression of the protein P26.Amplification and cloning of the DNA fragment isolated, insertion of the fragment in carreadores microorganisms using a vector of expression.A fragment of DNA which encodes the protein glutathione s - transferase (GST) of Schistosoma japonicum and the sites of restriction necessary for the process of fusion gene.And purification of the fusion protein thus obtained.