Embryonic cells or similar to stem cells produced by cross species nuclear transplantation in

摘要

"Embryonic cells or similar to stem cells produced by cross species nuclear transplantation in.A process is provided for the nuclear transfer that involves the transplantation of donor cell nuclei differentiated in u00f3ocitos enucleados of a different kind of cell donor.In combination with the cytoplasm and / or the mitocu00f3ndrias compatible (of the same species donor).The resulting nuclear transfer units are useful for the production of cells similar to embryonic stem cells isogu00eanicas,In particular, the embryonic cells or similar to stem cells isogu00eanicas of human beings.These cells are similar to embryonic or stem cells are useful for the production of the desired differentiated cells and for the introduction, removal or modification of genes as desired, for example.At specific sites of the genome of such cells by homologous recombination.These cells, which may contain a heterologous gene, are especially useful in the therapy of transplantation of cell and for the in vitro study of cellular differentiation.The efficiency of nuclear transfer is enhanced by the introduction of cytoplasmic or mitochondrial DNA compatible (same species or similar species of the cell or nucleus donors).In addition, the efficiency of nuclear transfer may also optionally be improved by genetic alteration of donor cells to inhibit apoptosis.By selecting a cell cycle specific and / or cultivating under conditions that enhance the growth and embryonic development.